Super Co-NTA Agarose Resin


Super Cobalt NTA Affinity Resin designed for affinity purification of polyhistidine tagged proteins. Cobalt ions are carefully loaded onto a 7.5 % cross-linked agarose matrix (medium particle diameter 40 μm) via a NTA coupled ligand to obtain a stable affinity matrix with the highest binding capacity for histidine residues (up to 10 mg/ml determined from E. coli cleared lysate). Other metal ions such as Ni2+, Zn2+, Fe3+, and Al3+ can also be used resulting in different affinities. If required, the cobalt ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix recharged with a different metal ion.

Specifications

Specificity: Polyhistidine tag

Matrix: 7.5 % cross linked agarose

Coupled ligand: Nitrilotriacetic acid (NTA)

Binding capacity: 30 mg/ml

Bead size: 32 – 60 µm (40 μm medium)

Flow rate: 0.25 – 1 ml/min (optimum), 6 ml/min (max.)

Maximum pressure: 72 psi

Buffer compatibility: Common aqueous buffers from pH 2 - 4

Cleaning buffer examples: 100 % methanol, 100 % ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30 % (v/v) acetonitrile

Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol at ambient temperature

Storage: Equilibration buffer at 2 -°C (short-term) 20 % ethanol at 2-8 °C (long-term)




HS: 38229000


Cat.No.
Size
EUR
42322.01
100 ml
inquire*
Please Login to order.
*Please use the inquiry form in the right column.

PDF Documents

Product Inquiry
*
*
*
*Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

Promotions


Top

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.