2DHPE™ Mercator Gel 12.5 % Kit
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2D HPE™ Mercator Gels allow a much more precise XY-coordinates determination
of protein spots in 2D gels.
2D gel analysis is mostly done by
triangulation of the XY-coordinates of the protein spots of a 2D gel picture
with an appropriate software. For comparison of the spot pattern of different
gels, gels have to be normalized and the pictures are laid on top of each
other. A major obstacle in comparison to 2D protein gels are the technical
gel-to-gel variations because of differences in sample preparation, gel
casting and conduct of electrophoresis.
Simone König and her research group
(IZKF, Münster, Germany) developed a method for generation of a reference
grid, which improves the deviation of the correction of protein coordinates by
an order of magnitude: comparative 2D fluorescence gel electrophoresis
(CoFGE). The reference grid is formed by fluorescence-labelled marker
proteins, which are applied to the gel in equal distances. After
co-electrophoresis of protein marker and with a different fluorescence dye
labelled protein sample, the XY-coordinates are much more precisely determined
with the aid of the multiple triangulation points formed by the reference grid
compared to standard methods.
In cooperation with the research group of
Simone König, SERVA developed the 2D HPE™ Mercator Gels for CoFGE on
horizontal electrophoresis systems HPE™ BlueTower and HPE™ BlueHorizon™.
For
easy loading of the fluorescence-labelled protein marker, the gels have in
addition to the IPG slot 14 sample slots. The slots are on the cathode side in
a distance of 3 mm to the IPG slot; the distance to each other is 18 mm. The
kit contains 4 plastic-backed gels, including running and equilibration
buffers, FS wicks and cooling contact fluid.
References:
- Ackermann, D. et al. (2012) Electrophoresis 33, 1406-1410
- Hanneken, M. and König, S. (2014) Electrophoresis 35, 1118-1121
HS: 38220000
Storage Temperature: +2 °C to +8 °C