Activities: Pectinase: ca. 1 U/mg Unit definition: 1
U catalyzes the liberation of 1 µmole of reducing groups from pectic acid per
minute at 25 °C, pH 4.5 calculated as galacturonic acid. Reducing groups
determined with alkaline copper reagent (1). Hemicellulase: ca. 0.5
U/mg Unit definition: 1 U is equivalent to 1 µmole of reducing
groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as
xylose. Cellulase: ca. 0.1 U/mg Unit definition: 1 U
catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl
cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper
reagent (2). pH optimum: 3.5 - 7.0 Temperature optimum: 40
- 50 °C
Barraclough, R. & Ellis, R.J. (1979) Eur. J. Biochem. 94, 165-77
Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
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