IEF separates proteins in their native state. When transferred onto membranes, proteins feature full antigenicity and can be detected by specific antibodies. Moreover, the blotted proteins may be subjected to enzymatic reactions to further assess functional pathways.Blotting of gels bound to support film cannot be performed without removal of the film backing (film is not permeable in electroblotting). But IEF gels are very thin and carry the risk to tear when being handled in blotting procedures.
SERVA developed the SERVALYT™ PreNets™ particularly for use in blotting
experiments and assays which assess enzymatic activity of gel separated
proteins. They are precast gels, used in the same manner as the related
SERVALYT™ PRECOTES™ except that the gel, supported by the fabric, is permeable
for electrotransfer. The gel layer is not covalently bound to the backing and
is lifted off easily.