Blank PRECOTES™ and Blank PreNets™ were developed by SERVA to provide a versatile solution to perform isoelectric focusing (IEF) of any pH range. Blank PRECOTES™ are thin (0.3 mm) polyacrylamide gels cast onto GEL-FIX™ support film that contain only BisTris buffer pH 6.5; Blank PreNets™ are cast onto Net-Fix™ to facilitate blotting.
They are given the prefix »blank« to indicate that they are (almost) »empty« gels with a matrix that can be adapted to anything the user wants it to be. To perform IEF, Blank PRECOTES™/PreNets™ are equilibrated in the ampholyte mixture of choice for 30 minutes prior to electrophoresis. Resolution is at least as good, sometimes found even superior, compared to the results obtained with gels of »cast-in« ampholytes.
- can be adapted to any pH range desired by rapid equilibration
- suited for IEF in the basic pH range
- suited for IEF in the presence of urea
- excellent resolution
- long shelf life
- blotting gels available