Isoelectric Focusing

Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.

For IEF, SERVA offers a comprehensive product line:

  • SERVALYT™ Carrier Ampholytes
  • SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
  • FocusGels Precast Horizontal Gels
  • SERVAGel™ IEF Vertical Gels
  • Protein Standards
  • Buffers and Accessories
  • Equipment


News

New !

SERVA Catalog
2018

Product of the Month February:

BlueVertical™ PRiME™

Western Blotting -
New Brochure!

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New !

SERVA Catalog
2018

In summary here is a short description of products made available for the research community in 2018:
• Protein extraction kits for rapid isolation of membrane, cytoplasmic and nuclear proteins from mammalian tissues and cells
• Cell-based assays for apoptosis and cell profliferation/viability like XTT or Sulforhodamine B
• Enzymes like recombinant PNGase F or Lysyl Endopeptidase • Ultrapure reagents for UHPLC-MS analysis like acetonitrile and water
• Biochemicals for nucleic acid purification like recombinant Proteinase K or BlueZol for RNA isolation
• Electrophoresis gel and blot documentation: SERVA Musketeer is an all-in-one solution for UV/VIS, fluorescence and chemiluminescence applications

Download our new catalog here:

 

SERVA Catalog 2018 (pdf)
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Product of the Month February:

BlueVertical™ PRiME™

Electrophoresis Unit for Vertical Mini Gels

This way to the Special Offer

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Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting (pdf)
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