Lubrol 17A17 is a polyoxyethylene linear alkyl ether produced by ethoxylation of cetyl-stearyl alcohols.

It is a solid non-ionic surfactant and O/W emulsifier supplied in flakes. In cell biology, it is above all used for investigation on lipid rafts, the cholesterol- and glycosphingolipid-rich membrane areas, which play an important role in many cellular processes, e.g. signal transduction. Furthermore it is used for cell and tissue lysis, for the isolation and characterization of membrane-bound enzymes, transporter proteins, and in some applications for column chromatography of membrane components.

Synonymes Brij™ CS17; Lubrol W1, Lubrol WX

CAS registry number: [68439-49-6]

Classification: Nonionic surfactant

HLB 15

MP 34 – 38 °C

Hydroxyl number 53.0– 60.0

pH 1% in water 7.0 – 9.0

Cloud point >70 °C

Bibliography

Isolation and Investigation of Lipid Rafts

Röper, K. et al. (2000) Retention of prominin in microvilli reveals distinct cholesterol-based lipid microdomains in the apical plasma membrane. Nature Cell Biology 2, 582-92.

Chamberlaine, L.H. et al. (2001) SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis. PNAS 98, 5619-24.

Mitsutake, S. et al. (2001) Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney. J. Biol. Chem. 276, 26249-59.

Kalus, I. et al. (2002) Interaction of syncollin with GP-2, the major membrane protein of pancreatic zymogen granules, and association with lipid microdomains. Biochem. J. 362, 433-42.

Sakyo, T. & Kitagawa, T. (2002) Differential localization of glucose transporter isoforms in non-polarized mammalian cells: distribution of GLUT1 but not GLUT3 to detergent-resistant membrane domains. BBA Biomembranes 1567, 165-75.

Schuck, S. et al. (2003) Resistance of cell membranes to different detergents. PNAS 100, 5795-800.

Slimane, T.A. et al. (2003) Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role  of distinct lipid microdomains. Mol. Biol. Cell 14, 611-24.

Vetrivel, K.S. et al (2004) Association of -secretase with lipid rafts in post-golgi and endosome membranes. J. Biol. Chem. 279, 44945-54

Müller, G. et al. (2005) Regulation of lipid raft proteins by glimepiride- and insulin-induced glycosylphosphatidylinositol-specific phospholipase C in rat adipocytes. Biochem. Pharmacol. 69, 761-80

Florek, M. et al. (2007) Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine. Cell and Tissue Research 328, 31-47.

Sturm, A.K. et al. (2007) Functional expression and microdomain localization of prestin in cultured cells. Otolaryngal Head Neck Surg. 136, 434-9.

Garner, A.E. et al. (2008) Visualization of detergent solubilization of membranes: implications for the isolation of rafts. Biophys. J. 94, 1326-40.

Huttner, H.B. et al. (2008) The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease. Stem Cells 26, 698-705.     

Sakurai, T. et al. (2008) Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J. Biol. Chem. 183, 339-52.

Gersbacher, M.T. er al. (2010) Identification of BACE1 cleavage sites in human voltage-gated sodium channel beta 2 subunit. Molecular Neurodegeneration 5, 61

Rajagopalan, L. et al. (2010) Glycosylation regulates prestin cellular activity. J. Assoc. Res. Otolaryngol. 11, 39-51.

Woudenberg, J. et al. (2010) Lipid rafts are essential for peroxisome biogenesis in HepG2 cells. Hepatology 52, 623-33.

Cell and Tissue Lysis

Mairhofer, M. et al. (2002) Stomatin is a major lipid.raft component of platelet  granules. Blood 100, 897-904

Slimane, T.A. et al. (2003) Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role  of distinct lipid microdomains. Mol. Biol. Cell 14, 611-24.

Morrison, H. et al. (2007) Merlin/neurofibromatosis type 2 suppresses growth by inhibiting the activation of Ras and Rac. Cancer Res. 67, 520-7.

Paul, V. et al. (2008) A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J. Lipid Res. 49, 2259-67.

Isolation of Membrane-bound Enzymes and Proteins

Rafael, J. (1983) Cytochrome c Oxidase. In: Methods in Enzymatic Analysis, vol. 3 (Bergmeyer, H.U., ed.), pp. 266-73, Verlag Chemie, Weinheim.

Cunningham, S.A. a. Nicholls, D.G. (1987) Induction of functional uncoupling protein in guinea pigs infused with noradrenaline. Biochem. J. 245, 485-491
Fricke, B. et al.(1993) Membrane-bound proteinases of Halobacterium halobium. J. Basic Microbiology 33, 9-18

Chromatographic Separations

Mitsutake, S. et al. (2001) Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney. J. Biol. Chem. 276, 26249-59.