Product of the Month

Proteinase K, NGS Grade

Proteinase K from Tritirachium album expressed recombinantly in Pichia pastoris

Proteinase K is a subtilisin-like serine protease with a broad substrate specificity. The enzyme degrades both terminal (exopeptidase activity) and internal (endopeptidase activity) peptide bonds.

The enzyme is active under wide range of reaction conditions, including elevated temperatures and presence of SDS. This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

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Features

  • Integrity of the isolated nucleic acids remains intact
  • Contains no endo-, exo- and ribonucleases
  • Solubility in water: ≥ 50 mg/m
  • DNA content: ≤ 0.1 pg/mg enzyme
  • Serin protease
  • Increased stability by Ca2+ (1 - 6 mM)
  • Activated by 0.2 - 1 % (w/v) SDS, 4 M urea
 
  • Active in the ph range: 4.0 – 12.0
    (optimum: 7.5 – 8,.)
  • Active in the temperature range 20 °C – 65 °C (maximum activity at 50 °C – 65 °C)
  • Inhibition by the following serine protease inhibitors: AEBSF, PMSF and DFP
  • Unaffected by metal-chelating agents and sulfhydryl inhibitors

Ordering Information:

Product
 Cat. No.
Size
Price
Special Price*
Proteinase K recombinant,
min. 35 mAnson-U/mg, NGS grade
33757.01
33757.02
33757.03
  25 mg
100 mg
500 mg
  43.00 €
113.00 €
509.00 €
  34.40 €
  90.40 €
407.20 €
*The special price is valid until November 30th,  2021  
Please Note:
The "Product of the Month" promotion is for all SERVA customers within the EU and cannot be combined with other discounts, promotions etc.

Here you will find further sample preparation products, e.g

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Product of the Month November (PDF)  

 


Problems with 2D electrophoresis?

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If you have any further questions, please contact the SERVA team by phone (+49 6221 138 40-44) or by e-mail (Tech.Service@serva.de). 

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New !

SERVA Catalog
2021

In detail you will find the following new products in catalog 2021:

  • • New enzymes like dsDNase for highly specific degradation of doubled-stranded DNA
  • • Zymolyase® to produce yeast protoplast or spheroplast
  • • Tergitol™ as an alternative to Triton X-100
  • • Easy Peel 2D HPE™ gels for blotting of horizontal 2D gels
  • • New protein standards, e.g. SERVA Unstained Protein Standard 6.5 - 97 kDa
  • • BlueVertical™ PRiME™ Blot Modul for SERVA’s BV-104 electrophoresis tank
  • • IPG tray with electrode lid for 1st dimension protein separation on 7 cm to 24 cm IPG strips
  • • Special Pharma Edition of SERVA BlueStain automated gel staining device

 

SERVA Catalog 2021 (pdf)
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Now select the lot number from the list and download the PDF with a click. Done.

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Western Blotting -
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  • • Molecular Weight Standards
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SERVA Brochures: Western Blotting V2110 (pdf)
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