Product of the Month June

BlueBlock PF (10x) for Blotting

Protein-free Blocking Reagent for ELISA & Western Blots

To achieve a good signal-to-noise ratio, it is important to block non-specific antibody binding sites on the transfer membrane.
Protein based blocking solutions like skim milk, BSA or casein solutions may not only block unspecific binding sites, but mask as well specific binding sites.
With BlueBlock PF (protein-free, polymer-based), the specific binding sites remain accessible while non-specific reactions are suppressed, thus leading to an increase in signal intensity.

It is suitable for colorimetric and chemiluminescence detection systems.

BlueBlock.jpg

How to use BlueBlock PF:

 Dilution
Dilute the 10x concentrate with dist. water to get a 1x solution,
e.g. 10 ml BlueBlock PF + 90 ml dH2O
Mix well
 Blocking
1 h incubation of the membrane with 1x solution at RT on an orbital shaker
Optional: at + 2 °C to  + 8 °C over night
 1. Ab Incubation
Dilute the antibody (Ab) with 1x BlueBlock PF
1 h incubation of the membrane at RT on an orbital shaker
 Wash
3 x 5 min with PBS-T or TBS-T
 2. Ab Incubation
Dilute the Ab with 1x BlueBlock PF
1 h incubation of the membrane at RT on an orbital shaker
 Wash
3 x 5 min with PBS-T or TBS-T 
 Detection
Using the appropriate substrate

 

Ordering Information:

 Product
 Cat. No.
Size
Price
Special Price*
 BlueBlock PF (10x) for Blotting
 42591.01
42591.02
250 ml
1 L
   41.00 €
128,00 €
  32.80 €
102.40 €
 
*The special price is valid until June  31st, 2018

Please Note:
The "Product of the Month" promotion is for all SERVA customers within the EU and cannot be combined
with other discounts, promotions etc.

 

Promotions

Product of the Month June:

BlueBlock PF (10x)
for Blotting

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Product of the Month June:

BlueBlock PF (10x)
for Blotting

The Protein-free Blocking Reagent for ELISA & Western Blots

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