Ampicillin·Na-saltmolecular biology grade, Ph. Eur.

(aminophenylacetamido]penicillanic acid, aminobenzylpenicillin)
C16H18N3O4S·NaMr 371.4CAS [69-52-3]

Ampicillin sodium salt is a semi-synthetic derivative of penicillin used to select for ampicillin resistance in mutated and transformed cells. Ampicillin is a ß-lactam antibiotic that inhibits bacterial cell wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane. The antimicrobial spectrum includes gram-positive and gram-negative bacteria.
Recommended for antibacterial use in cell culture media at 100 µg/ml and in ampicillin-resistance studies at 20 - 125 μg/ml.
Solubility in water: 1 part in 2 parts H2O. A stock solution should not be autoclaved but sterilized through filtration and stored frozen where it will be stable for months. Stability of ampicillin in solution is a function of pH, temperature and the identity of the buffer. Optimal storage conditions are 2 - 8 °C and pH 3.8 - 5 where its activity is retained at >90 % for a week.

Assay
91.0 - 102.0 %


References:
  1. Davies, J. & Smith, D.J. (1978) Ann. Rev. Microbiol. 32, 469
DANGER
Hazard Statements H317 - H334
Precautions P280 - P285
Reaction P302 +P352 - P304 +P341 - P333 +P313 - P342 +P311

EINECS: 200-708-1WGK: 1HS: 29411000
Storage Temperature: +2 °C to +8 °C

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H317

Deutsch:

Kann allergische Hautreaktionen verursachen.

English:

May cause an allergic skin reaction.

Français:

Peut provoquer une allergie cutanée.

Italiano:

Può provocare una reazione allergica cutanea.

Español:

Puede provocar una reacción alérgica en la piel.

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P333 +P313

Deutsch:

Bei Hautreizung oder -ausschlag: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

English:

If skin irritation or rash occurs: Get medical advice/attention.

Français:

En cas d’irritation ou d'éruption cutanée: consulter un médecin.

Italiano:

In caso di irritazione o eruzione della pelle: consultare un medico.

Español:

En caso de irritación o erupción cutánea: Consultar a un médico.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.