Pronase E from Streptomyces griseus min. 5.0 DMC-U/mglyophil.

(Streptomyces griseus neutral proteinase, Actinase E)
CAS [9036-06-0]

Mixture of at least 10 proteases: five serine type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases and one zinc carboxypeptidase. Digestion with the product has been useful when extensive or complete degradation of protein is required. Pronase E is used in tissue dissociation of various tissues, e.g. to isolate living chondrocytes.
Additional applications are the structural analysis of proteins (1, 2), preparation of bacteriophage lambda DNA (3), pretreatment of tissue sections to enhance the intensity of immunostaining and removal of protein in DNA/RNA isolations.

Activity: min. 5.0 DMC U/mg Unit definition: 1 DMC-U (1U) is that amount of enzymatic activity which catalyzes the cleavage of 1 µ-equivalent peptide bond from dimethyl casein per minute at 25 °C, pH 7.5, expressed in terms of the appearance of new terminal amino groups (4).
Activity in other units: ca. 1 000 000 PU-units/g (casein substrate; 40 °C, pH 7.4 (5), ca. 20 000 PUK-units/g (casein substrate, 40 °C, pH 7.5).
Pronase = registered trademark of Calbiochem-Novabiochem Corp.


References:
  1. Jehanli, A. & Hough, D. (1985) Mol. Immunol. 22, 557-66
  2. Tsugita, A. & Akabori, S. (1959) J. Biochem. (Tokyo) 46, 695-704
  3. Maniatis, T. et al. (1982) Molecular Cloning - a Laboratory Manual. Cold Spring, Harbor Laboratory, p. 85
  4. Lin, Y. et al. (1969) J. Biol. Chem. 244, 789-93
  5. Nomoto, M. & Narashi, Y. (1959) J. Biochem. (Tokyo) 46, 653-67
DANGER
Hazard Statements H315 - H319 - H334 - H335
Precautions P280 - P285
Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-014-00-9EINECS: 232-909-5WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

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H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

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H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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H335

Deutsch:

Kann die Atemwege reizen.

English:

May cause respiratory irritation.

Français:

Peut irriter les voies respiratoires.

Italiano:

Può irritare le vie respiratorie.

Español:

Puede irritar las vías respiratorias.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

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P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.