2DHPE™ Triple BlotGel NF 12.5 % KitSize: 250 x 110 x 0.65 mm
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The 2D HPE™ BlotGels are best suited for high resolution separation of
proteins by 2D gel electrophoresis and subsequent blotting for diiferent
applications, especially for host cell protein (HCP) analysis by 2D Western
immuno blotting. The non-fluorescent (NF) supporting film also provides best
results for fluorescent staining and labelling.
Analysing large-format, horizontal 2D gels by immunodetection has not been possible until now, or only under very difficult conditions. The carrier foil, which is necessary to support the gel during all handling processes in 2D gel electrophoresis, makes a semi-dry blot impossible. Until now, the separation of gel matrix and carrier foil was only possible by a laborious mechanical procedure, which often resulted in the gel with the separated samples being more or less destroyed.
SERVA has found a simple trick to circumvent this dilemma. In the 2D HPE™ blot gels, the gel matrix is covalently bonded to the carrier foil via a narrow strip at the edge, while the majority of the gel only adheres non-covalently to the foil. Before transferring the proteins to the membrane by means of semi-dry western blotting, the covalently bound strip can be cut off and the gel easily separated from the carrier foil.
Kit comprising 4 plastic-backed gels, including running and equilibration buffers, FS wicks and cooling contact fluid.
Suitable for running 3 x 7 cm IPG strips plus 1 marker lane by horizontal electrophoresis on HPE™ BlueTower, HPE™ BlueHorizon or Multiphor II™.
Storage Temperature: +2 °C to +8 °C