PNGase F, recombinantlyophilized
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Mr ca. 36.000 •
Recombinant glycosidase from Flavobacterium meningosepticum with faster
kinetics and greater activity against native glycoproteins.
The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources:
Catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
Concentration after reconstitution: 1000 u/µl (2.0 mg/ml) in 50 µl H2O dest.
Unit definition: Achieves complete deglycosylation of 10 µg of RNase B incubated in 1x PBS with 1 µl of PNGase F for 5 - 10 min at 37 °C or room temperature.
Storage Temperature: +15 °C to +30 °C