Lysozyme from chicken egg white min. 15 000 units/mgcryst.

(Muramidase, Mucopeptide-glycohydrolase, Mucopeptide N-acetyl-muramoylhydrolase)
EC 3.2.1.17Mr ca. 14 400 CAS [12650-88-3]

Lysozyme hydrolyzes the β 1 → 4 linkages of the murein between N-acetylmuramic acid and N-acetyl-D-glucosamin, and degrades the heteroglycan chain to disaccharides. This reaction leads to cell lysis in most gram positive bacteria e.g. Micrococcus luteus. Lysis can be prevented if the reaction is performed in an isotonic sucrose medium. Under these assay conditions protoplasts are produced which no longer have a cell wall. The lysis of Micrococcus luteus cells is used for the activity testing of lysozyme. In gram negative bacteria a compact lipopolysaccharide layer on the exposed murein sacculus efficiently shields them from lysozyme digestion. Only when the stabilizing Ca2+ ions are removed by treatment with e.g. EDTA, the murein becomes susceptible to lysozyme. Suitable for hydrolysis of bacterial cell walls and of proteoglycans (1, 2).



Unit definition: 1 unit catalyzes a decrease in absorption at 450 nm of 0.001 per minute at 25 °C, pH 6.24 using a suspension of Micrococcus lysodeikticus as substrate.


References:
  1. Imoto, T. et al. (1972) The Enzymes VII, 3rd Ed. (Boyer, P.D., ed.) Acad. Press N.Y. 666-70
  2. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (1.22, 1.34, 1.36, 1.38, 17.38, 1.29, B.17)
DANGER
Hazard Statements H334
Precautions P261 - P284
Reaction P304 +P340 - P342 +P311

EINECS: 235-747-3WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P284

Deutsch:

Atemschutz tragen.

English:

Wear respiratory protection.

Français:

Porter un équipement de protection respiratoire.

Italiano:

Utilizzare un apparecchio respiratorio.

Español:

Llevar equipo de protección respiratoria.

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P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.