IEF Marker 3-10, Liquid Mix

(Protein Standards, Protein marker, IEF Marker, IEF Standard)

The ready-to-use protein standard for isoelectric focusing (IEF) enables reliable and reproducible calibration of the isoelectric point (pI) of native proteins in polyacrylamide or agarose IEF gels. IEF Marker 3-10 contains nine native proteins in the pI range 3.5 to 10.7 (13 isoforms). The naturally red coloured cytochrome C facilitates monitoring of the focusing. In addition, the two dyes bromophenol blue and methyl red indicate migration progress. Bromophenol blue changes from blue to yellow as soon as it reaches the anodic region below pH 3. Methyl red migrates as a yellowish band that turns red below pH 6 and concentrates as a distinct band at pH 3.8. The dyes are removed from the gel during fixation/staining.
Staining with SERVA Violet 17 (cat. no. 35072 and 35074), SERVA Blue G (cat. no. 35050), SERVA Blue R (cat. no. 35051): 5 µl/lane for horizontal gels, 5 µl/lane for vertical gels
Silver staining: 1:10 dilution and 5 µl/lane

Buffer composition: 0.01 % Bromophenol blue (Na-salt), 0.01 % Methyl red (Na-salt), 10 %

Amyloglucosidase
Glucose oxidase
Trypsin inhibitor (soybean)
β-Lactoglobulin
Carboanhydrase
Myoglobin (horse heart)
Lentil lectin
Ribonuclease A
Cytochrome C

pI 3.5
pI 4.2
pI 4.5
pI 5.15/5.3
pI 6.0
pI 6.9/7.35
pI 7.75/8.0/8.3
pI 9.45
pI 10.65


HS: 38229000
Storage Temperature: -15 °C to -25 °C

Cat.No.
Size
EUR
39212.01
500 µl
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2023

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
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• Isoelectric Focusing

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