3,3'-Diaminobenzidine·4HCl·xH2Oresearch grade
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(DAB, Diaminobenzidine tetrahydrochloride)
C12H14N4·4HCl·xH2O • Mr 360.1 (anhydr.) • CAS [868272-85-9]
C12H14N4·4HCl·xH2O • Mr 360.1 (anhydr.) • CAS [868272-85-9]
3,3'-Diaminobenzidine tetrahydrochloride (DAB) is a substrate for peroxidases, especially for horseradish peroxidase (HRP).
DAB is used for histology and ultrahistochemistry (1) and demonstration of ultrastructural peroxidase (2).
It serves as a hydrogen donor in the presence of peroxide in the peroxidase reaction. The oxidized DAB forms an insoluble brown end-product, which can be detected in visible light and does not bleach during long-term storage.
Stock solution: 10 - 20 mg/ml in 50 mM Tris buffer, pH 7.3 (store in aliquots at -20 °C), working solution: 0.5 - 1 mg/ml in buffer (PBS, TBS, pH 7.0 - 7.6) containing 0.01 % hydrogen peroxide.
Vials under argon.
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Assay (HPLC)
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min. 96.0 %
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References:
- Hanker, J.S. et al. (1972) Histochemie 30, 201-14
- Graham, R.C. & Karnovsky, M.J. (1966) J. Histochem. Cytochem. 14, 291- 302
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| DANGER |
| Hazard Statements | H341 - H350 |
| Precautions | P201 - P280 |
| Reaction | P308 +P313 |
| Storage | P405 |
Carc. 1B, Muta. 2 • EINECS: 231-018-9 • WGK: 3 • HS: 29215990
Storage Temperature: +2 °C to +8 °C
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