Cellulase »Onozuka« RS from Trichoderma viride ca. 2 U/mg

CAS [9012-54-8]

A multi-component enzyme system (1). Although the preparation has high cellulase activity (EC 3.2.1.4), it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Contains about three times as high xylanase activity as Cellulase Onozuka R-10 (cat. no. 16419). Widely used for the isolation of protoplasts, for its ability to degrade cell walls, often in combination with Macerozyme R-10 (cat. no. 28302) (2).
Temperature optimum: 50 - 60 °C
pH-optimum: pH 4 - 5

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Extraneous activities: Contains α-amylase, pectinase, protease and hemicellulase.


References:
  1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
  2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
  3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
  4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EG-Index: 647-002-00-3EINECS: 232-734-4WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
16420.01
1 g
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16420.02
5 g
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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.