(NBT, Nitro BT, Nitrotetrazolium blue chloride, Ditetrazolium dye) C40H30Cl2N10O6 • Mr 817.7 • CAS [298-83-9]
A ditetrazolium compound for demonstrating succinic dehydrogenase activity
(1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). Used in
conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase. Stock
solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C. Staining
solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock
solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM
Tris; pH 9.5)
min. 98.0 %
Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
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