Nitro blue tetrazolium chloride

analytical grade

(NBT, Nitro BT, Nitrotetrazolium blue chloride, Ditetrazolium dye)
C40H30Cl2N10O6Mr 817.7CAS [298-83-9]

A ditetrazolium compound for demonstrating succinic dehydrogenase activity (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). Used in conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase.
Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.
Staining solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)

Purity
min. 98.0 %


References:
  1. Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
  2. Negi, D.S. & Stephens, R.J. (1977) J. Histochem. Cytochem. 25, 149-54
  3. Auscher, C. & Amory, N. (1976) Biomedicine 5, 37-8
WARNING
Hazard Statements H302 - H332
Precautions P261
Reaction P301 +P312 - P304 +P340 - P330
Storage P501

EINECS: 206-067-4WGK: 1HS: 29339980
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
30550.01
250 mg
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30550.02
1 g
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30550.03
5 g
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H302

Deutsch:

Gesundheitsschädlich bei Verschlucken.

English:

Harmful if swallowed.

Français:

Nocif en cas d'ingestion.

Italiano:

Nocivo se ingerito.

Español:

Nocivo en caso de ingestión.

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H332

Deutsch:

Gesundheitsschädlich bei Einatmen.

English:

Harmful if inhaled.

Français:

Nocif par inhalation.

Italiano:

Nocivo se inalato.

Español:

Nocivo en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P301 +P312

Deutsch:

BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.

Français:

EN CAS D’INGESTION: appeler un CENTRE ANTIPOISON ou un médecin en cas de malaise.

Italiano:

IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI o un medico.

Español:

EN CASO DE INGESTIÓN: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico si se encuentra mal.

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P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P330

Deutsch:

Mund ausspülen.

English:

Rinse mouth.

Français:

Rincer la bouche.

Italiano:

Sciacquare la bocca.

Español:

Enjuagarse la boca.