Home › Collagenase NB 8 Broad Range from Clostridium histolyticum
Collagenase NB 8 Broad Range from Clostridium histolyticum
(Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Contains class I and class II collagenase (2) as well as reduced levels of
clostripain, trypsin-like activities and neutral protease. Chromatographically
purified. PZ activity (Wünsch): ≥ 0.90 U/mg Endotoxin: ≤ 100
EU/mg (Ph. Eur.) Suitable for a broad range of different applications in
tissue dissociation such as isolation of rodent pancreatic islets of
Langerhans, porcine hepatocytes, cardiomyocytes, tumor cells, muscle cells and
human osteoblasts. Increased PZ activity compared to Collagenase NB 4 Standard
Grade (cat. no. 17454) and Collagenase NB 4G Proved Grade (cat. no. 17465).
Unit definition: Collagenase: 1 U according to Wünsch (1)
catalyzes the hydrolysis of 1 µmole
4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per
minute at 25 °C, pH 7.1. Neutral Protease: 1 DMC Unit catalyzes
the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C,
pH 7.0, expressed in terms of newly formed terminal amino groups, determined
with TNBS. Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1
International Unit of a WHO approved reference standard endotoxin (RSE)).
References:
Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
Proteomics in Practice (Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt. Electrophoresis in Practice Reiner Westermeier): 109,00 € inkl. 7 % MWSt.
Proteomics in Practice: (HPE-PIP) Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Electrophoresis in Practice: (HPE-EIP) Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.
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BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.
