Home › Collagenase NB 1 GMP Grade from Clostridium histolyticum
Collagenase NB 1 GMP Grade from Clostridium histolyticum
(Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Contains chromatographically highly purified class I and class II collagenase. Largely
free from clostripain, trypsin-like protease and neutral protease. Vial
contains not less than 2000 PZ U collagenase. PZ activity (Wünsch): ≥
3.00 U/mg Endotoxin ≤ 10.0 EU/mg (Ph. Eur.) Microbiological
examination: Total aerobic microbial count (TAMC), Total combined yeast/moulds
count (TYMC), Absence of Clostridia, Abnormal toxicity
(General-Safety-Test; Ph. Eur.). Manufactured according to EU eGMP
guidelines. This highly purified collagenase quality is used for isolation
of sensitive cells and guarantees high yields and viability. In combination
with Neutral Protease NB GMP Grade (cat. no. 30303) Collagenase NB 1 GMP Grade
is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase
NB 1 GMP Grade is recommended for applications which have to meet cGMP
requirements like tissue engineering and transplantation into humans.
Unit definitions: Collagenase: 1 U according to Wünsch (1)
catalyzes the hydrolysis of 1 µmole
4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl- L-prolyl-D-arginine per
minute at 25 °C, pH 7.1. Endotoxin: Ph. Eur. (1 Endotoxin Unit
is equal to 1 International Unit of a WHO approved reference standard
endotoxin (RSE)).
References:
Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
Proteomics in Practice (Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt. Electrophoresis in Practice Reiner Westermeier): 109,00 € inkl. 7 % MWSt.
Proteomics in Practice: (HPE-PIP) Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Electrophoresis in Practice: (HPE-EIP) Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.
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