Proteinase K from Tritirachium album min. 30 mAnson-U/mglyophil.

EC 3.4.21.14Mr ca. 28 000CAS [39450-01-6]

Serin protease with very broad range of action: cleaves peptide bonds at the carboxylic side of aliphatic, aromatic, and hydrophobic amino acids. Suitable for the isolation of DNA and RNA (1, 3).

Unit definition: 1 mAnson unit is defined as the amount of enzyme that liberates folin-positive amino acids and peptides, corresponding to 1 µmol tyrosine per minute at 37 °C and pH 7.4 using urea-denatured hemoglobin as substrate (4).
Inhibitors for proteinase: AEBSF (cat. no. 12745), (PEFABLOC® SC (cat. no. 31682) and PMSF (cat. no. 32395)
Extraneous activities: DNases and RNases not detectable


References:
  1. Ebeling, W. et al.(1974) Eur. J. Biochem. 47, 91-7
  2. Lin, Y. et al.(1969) J. Biol. Chem. 244, 789-93
  3. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.16, 1.61)
  4. Anson, M.L. (1938) J. Gen. Physiol. 33, 79-89
DANGER
Hazard Statements H315 - H319 - H334 - H335
Precautions P280 - P284
Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-014-00-9EINECS: 254-457-8WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
33752.01
25 mg
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33752.02
100 mg
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33752.03
500 mg
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H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

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H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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H335

Deutsch:

Kann die Atemwege reizen.

English:

May cause respiratory irritation.

Français:

Peut irriter les voies respiratoires.

Italiano:

Può irritare le vie respiratorie.

Español:

Puede irritar las vías respiratorias.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P284

Deutsch:

Atemschutz tragen.

English:

Wear respiratory protection.

Français:

Porter un équipement de protection respiratoire.

Italiano:

Utilizzare un apparecchio respiratorio.

Español:

Llevar equipo de protección respiratoria.

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P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

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P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.