M9-Minimal salts 5x, powder52.5 g for 1 liter medium


For cultivation of E. coli and plasmid amplification in molecular biology
30 g/l Na2HPO4
15 g/l KH2PO4
5 g/l NH4Cl
2.5 g/l NaCl
For making 1 L 5x concentrate dissolve 52.5 g in 1 L distilled water and sterilize by autoclaving. The 5x concentrate can be stored and diluted as needed to prepare 5 L of 1x M9 minimum salts.


References:
  1. Sambrook, J., et al., Molecular Cloning : A Laboratory Manual, 2nd ed., p. A.3, Cold Spring Harbor laboratory Press, Cold Spring Harbor, New York

HS: 38210000
Storage Temperature: +15 °C to +30 °C

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