Macerozyme R-10® from Rhizopus sp.lyophil.

(»Macerating Enzyme«, »Cell Separating Enzyme«, Pectinase)
EC 3.2.1.15CAS [9032-75-1]

Macerozyme R-10® can be used in combination with cellulase "Onozuka R-10®" (cat. no. 16419) (1, 2) or with cellulase "Onozuka RS®" (cat. no. 16420) for the isolation of plant cells and especially for the isolation of metabolically active protoplasts.

Activity:
Pectinase: approx. 0.5 U/mg; unit definition: 1 unit catalyses the release of 1 µMol of reducing groups from pectic acid per minute at 25 °C, pH 4.5, calculated as galacturonic acid. Reducing groups are determined with an alkaline copper reagent (2).
Hemicellulase: approx. 0.25 U/mg; unit definition: 1 unit corresponds to 1 µMol of reducing groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as xylose.
Cellulase: approx. 0.1 U/mg; unit definition: 1 unit catalyses the release of 1 µMol glucose from sodium carboxymethylcellulose per minute at 40 °C, pH 4.5; glucose is determined with an alkaline copper reagent (3).
Optimum pH: 3.5 - 7.0
Optimum temperature: 40 - 50 °C.
Macerozym R-10® is a registered trademark of Yakult Pharmaceuticals Industry Co., Ltd..


References:
  1. Yamada, Y. et al. (1972) Agr. Biol. Chem. 36, 1055-9
  2. Barraclough, R. & Ellis, R.J. (1979) Eur. J. Biochem. 94, 165-77
  3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EINECS: 232-885-6WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
28302.02
2.5 g
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28302.03
10 g
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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.