Home › Lysozyme from chicken egg white min. 100 000 units/mg
Lysozyme from chicken egg white min. 100 000 units/mg
cryst.
(Muramidase, mucopeptide-glycohydrolase, mucopeptide N-acetyl-muramoylhydrolase) EC 3.2.1.17 • Mr ca. 14 400 • CAS [12650-88-3]
Crystalline powder in hydrochloride form. Lysozyme hydrolyzes the β 1 → 4
linkages of the murein between N-acetylmuramic acid and N-acetyl-D-glucosamin,
and degrades the heteroglycan chain to disaccharides. This reaction leads to
cell lysis in most grampositive bacteria e.g. Micrococcus luteus. Lysis
can be prevented if the reaction is performed in an isotonic sucrose medium.
Under these assay conditions protoplasts are produced which no longer have a
cell wall. The lysis of Micrococcus luteus cells is used for the
activity testing of lysozyme. In gramnegative bacteria a compact
lipopolysaccharide layer on the exposed murein sacculus efficiently shields
them from lysozyme digestion. Only when the stabilizing Ca2+ ions
are removed by treatment with e.g. EDTA, the murein becomes susceptible to
lysozyme. Suitable for hydrolysis of bacterial cell walls and of proteoglycans
(1, 2, 4).
Unit definition: 1 unit catalyzes a decrease in absorption at 450 nm of
0.001 per minute at 25 °C, pH 7.0, in a suspension of Micrococcus
luteus cells (reaction volume 3 ml, light path 1 cm) (3). Isoelectric
point: 10.5 - 11.0 Optimum pH: 9.2
References:
Imoto, T. et al. (1972) The Enzymes VII, 3rd Ed. (Boyer, P.D., ed.) Acad. Press N.Y. 666-70
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H334
Deutsch:
Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.
English:
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Français:
Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.
Italiano:
Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Español:
Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.
BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position
ruhigstellen, die das Atmen erleichtert.
English:
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
Français:
EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.
Italiano:
IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
Español:
EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.
