Proteus NoEndoµ (Micro) 2 Column Kit


Residual endotoxin contamination in advanced biotherapy products is an expensive and often difficult contaminant to control.

Many commercially available protocols are unable to remove endotoxins effectively and are based on non-affinity chromatography methods e.g. ion exchange chromatography, phase separation using Triton X-114 or require time consuming and expensive affinity steps. These costly resins are often supplied as loose resin or packed in slow gravity columns.

The Proteus NoEndo™ spin column kits offer a standardised method for high grade clearance of endotoxin from recombinant proteins, antibodies and viral vectors. These agents are increasingly being designed for therapeutic applications, hence moving them forward efficiently through in vivo studies requires pure preparations of the samples.

NoEndo™ μ spin columns incorporate our proprietary and NASA-inspired SelfSeal ™ membrane technology. The membrane is specially formulated to prevent any sample from leaking into the collection tube on an orbital mixer. In a centrifuge, the membrane pores dilate and the eluate, free of endotoxin, passes into the collection tube. The contact time is maximized to ensure maximum endotoxin depletion without losses of the target protein, antibody or domain antibody. Uniquely, there is also no dilution of the sample.




HS: 38229000


Cat.No.
Size
EUR
42242.01
1 kit
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