Glucose oxidase from Aspergillus niger min. 220 U/mglyophil.

(GOD)
EC 1.1.3.4Mr ca. 160 000CAS [9001-37-0]

Glucose oxidase is used in the enzymatic determination of D-glucose in solution (1). Glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide. Horseradish peroxidase is then used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
Ca. 300 U/mg protein.

Unit definition: 1 U catalyzes the oxidation of 1 µmole glucose to glucuronic acid per minute at 25 °C, pH 7 coupled with peroxidase and o-dianisidine (1).
Extraneous activities: Amylase, saccharase and maltase less than 0.05 %; GOD/catalase min. 2000.


References:
  1. Kunst, A. et al. (1984) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 6, p. 178-85
  2. Tsuge, H. & Mitsuda, H. (1973) J. Biochem. (Tokyo) 73, 199-206
  3. Pazur, J.H. (1966) in Methods in Enzymology (Colowick, S.P. & Kaplan, N.O., eds.) Vol. IX, 82-7
  4. O'Malley, J.J. & Weaver, J.L. (1972) Biochemistry 11, 3527-32
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EINECS: 232-601-0WGK: 1LHS: 35079090
Storage Temperature: -15 °C to -25 °C

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.