The assay bases on the bichinchoninic acid method (1). Proteins reduce
alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with
Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly
proportional to protein concentration.
Fast and sensitive assay: linear detection range from 25 – 1000 µg protein/ml
Easy to use: contains ready-to-use reagents and protein standard
Compatible with many detergents
Less binding variation between different proteins than Bradford assay
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BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.