Single tube format assay kit for protein quantification. The assay bases on
the precipitation of proteins as insoluble dye complexes with acidic,
ethanolic amido black 10B solution (1,2). After precipitation the protein-dye
complexes are spinned down. The pellet is washed and resolubilized. The
thereby released dye amount is measured at 624 nm.
Precise, reproducible, reliable assay data
Completed in only 45 min.
No interference with detergents or reducing agents
Detection range starts as low as 2 µg protein.
References:
Schaffner W., Weissmann C., A rapid, sensitive, and specific method for the determination
of protein in dilute solution, Anal. Biochem. 1973; 65: 502-514.
Popov N., Schmitt M., Schulzeck S., Matthies H., Reliable micro method for
determination of the protein content in tissue homogenates, Acta Biol. Med.
Ger. 1975; 34 (9): 1441-1446.
Proteomics in Practice (Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt. Electrophoresis in Practice Reiner Westermeier): 109,00 € inkl. 7 % MWSt.
Proteomics in Practice: (HPE-PIP) Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Electrophoresis in Practice: (HPE-EIP) Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.
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BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.
