Home › Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mg
Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mg
lyophil.
(DNase I, Deoxyribonucleodepolymerase, Deoxyribonucleate 5'-oligo-nucleotide hydrolase)
EC 3.1.21.1 • Mr 31 000 • CAS [9003-98-9]
EC 3.1.21.1 • Mr 31 000 • CAS [9003-98-9]
Cleaves preferentially double-stranded DNA (in the presence of Mg++-ions single stranded DNA) in oligonucleotides with 5'-terminal phosphate groups.
Unit definition: 1 Kunitz unit catalyzes an increase in absorption of 0.001 at 260 nm per minute at 25 °C, pH 5.0 when acting on highly polymerized calf thymus DNA (1).
Activity: min. 3000 Kunitz units/mg lyophilisate
References:
- Kunitz, M. (1950) J. Gen. Physiol. 33, 349-62
- Laskowski, M. Sr. (1971) The Enzymes IV, 3rd Ed. (Boyer, P.D.ed.) Acad. Press N.Y. 289-311
- Moore, S. (1981) The Enzymes XIV, 3rd Ed.(Boyer, P.D. ed.) Acad. Press N.Y. 281-96
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.83, 10.6-10.12, 15.14, 15.16, 15.27-15.31, 13.24-13.25, 13.28-13.29)
EINECS: 232-667-0 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 18535.01 | 25 mg | inquire* | |
| 18535.02 | 100 mg | inquire* | |
| *Please log in to see your actual prices. | |||
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