Home › Collagenase NB 4G Proved Grade from Clostridium histolyticum
Collagenase NB 4G Proved Grade from Clostridium histolyticum
(Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr
ca. 70 000 - 120 000 (collagenase)
• CAS [9001-12-1]
Contains class I and class II collagenase and a balanced mix of neutral
protease, clostripain and trypsin-like activities.
PZ activity (Wünsch): ≥ 0.18 U/mg
Suitable for isolation of a broad variety of cell types, especially for
fibroblasts, cells from adipose tissue (e.g. ADSC’s), human and rodent
hepatocytes, stem cells, frog oocytes and epithelial cells. Function tested.
Unit definition: 1 U according to Wünsch (1) catalyzes the hydrolysis of
1 µmole
4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per
minute at 25 °C, pH 7.1.
References:
Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333,
149-51
Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
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