Home › Collagenase NB 1 Premium Grade from Clostridium histolyticum
Collagenase NB 1 Premium Grade from Clostridium histolyticum
(Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Contains chromatographically highly purified class I and class II collagenase. Largely
free from clostripain, trypsin-like protease and neutral protease. Vial
contains not less than 2000 PZ U collagenase. PZ activity (Wünsch): ≥
3.0 U/mg Endotoxin ≤ 10.0 EU/mg (Ph. Eur.) This highly
purified collagenase quality is used in isolation of sensitive cells and
guarantees high yields and viability. In combination with Neutral Protease NB
(cat. no. 30301) Collagenase NB 1 Premium Grade is especially applicable to
isolation of pancreatic islets of Langerhans from different species like human
or pig. For research use only. If cells are intended for transplantation
into humans, Collagenase NB 1 GMP Grade (cat. no. 17452) is recommended.
Unit definition: Collagenase: 1 U according to Wünsch (1)
catalyzes the hydrolysis of 1 µmole
4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl- L-prolyl-D-arginine per
minute at 25 °C, pH 7.1. Endotoxin: Ph. Eur. (1 Endotoxin Unit
is equal to 1 International Unit of a WHO approved reference standard
endotoxin (RSE)).
References:
Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
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