Chloramphenicolresearch grade, Ph. Eur.

(Chloromycetin, dichloro-N-[β-hydroxy-α(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol)
C11H12Cl2N2O5Mr 323.1CAS [56-75-7]

Chloramphenicol is an antibiotic, isolated from strains of Streptomyces venezuelae. It blocks bacterial protein synthesis by inhibiting the peptidyl transferase activity of the 50S ribosomal subunit. The antibiotic has a broad application range against gram positive and gram negative bacteria.
Chloramphenicol is used for bacterial selection and plasmid amplification in molecular biology applications and as a selection agent for transformed cells containing chloramphenicol reistance genes.
A stock solution opf 50 mg/ml in ethanol yield a clear, very faint yellow solution. For selection of chloramphenicol-resistant Escherichia coli a concentration of 30 - 50 µg/ml are typically used.

Assay (UV)
98.0 - 102.0 %


References:
  1. Wal, J. et al. (1978) J. Chromatogr. 145, 502-6
  2. Danzer, L.A. (1983) Clin. Chem. 29, 856-8
  3. Rüssel, H. (1978) Chromatographia 11, 341-3
  4. Long, K.S. & Porse, B.T. (2003) Nucl. Acids Res. 31,7208-15
  5. Izard, T. (2001) Protein Sci. 10, 1508-13
  6. Schwarz, S. et al. (2004) FEMS Microbiol. Rev. 28, 519-42
  7. Zhu, H. et al. (2011) Int. J. Agric. Biol. 13, 677-82
  8. Munshi, T. et al. (2013) PLOS ONE 8(3):e60143
DANGER
Hazard Statements H350 - H361
Precautions P201 - P202 - P280
Reaction P308 +P313
Storage P405

Carc. 1B, Repr. 2EINECS: 200-287-4WGK: 3HS: 29414000
Storage Temperature: +15 °C to +30 °C

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H350

Deutsch:

Kann Krebs erzeugen <Expositionsweg angeben, sofern schlüssig belegt ist, dass diese Gefahr bei keinem anderen Expositionsweg besteht>.

English:

May cause cancer <state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard>.

Français:

Peut provoquer le cancer <indiquer la voie d'exposition s'il est formellement prouvé qu'aucune autre voie d'exposition ne conduit au même danger>.

Italiano:

Può provocare il cancro <indicare la via di esposizione se è accertato che nessun'altra via di esposizione comporta il medesimo pericolo>.

Español:

Puede provocar cáncer <Indíquese la vía de exposición si se ha demostrado concluyentemente que el peligro no se produce por ninguna otra vía >.

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H361

Deutsch:

Kann vermutlich die Fruchtbarkeit beeinträchtigen oder das Kind im Mutterleib schädigen <konkrete Wirkung angeben, sofern bekannt> <Expositionsweg angeben, sofern schlüssig belegt ist, dass die Gefahr bei keinem anderen Expositionsweg besteht>

English:

Suspected of damaging fertility or the unborn child <state specific effect if known > <state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard>.

Français:

Susceptible de nuire à la fertilité ou au foetus <indiquer l'effet spécifique s'il est connu> <indiquer la voie d'exposition s'il est formellement prouvé qu'aucune autre voie d'exposition ne conduit au même danger>.

Italiano:

Sospettato di nuocere alla fertilità o al feto <indicare l'effetto specifico, se noto><indicare la via di esposizione se è accertato che nessun'altra via di esposizione comporta il medesimo pericolo>.

Español:

Se sospecha que perjudica la fertilidad o daña al feto <indíquese el efecto específico si se conoce><indíquese la vía de exposición si se ha demostrado concluyentemente que el peligro no se produce por ninguna otra vía>.

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P201

Deutsch:

Vor Gebrauch besondere Anweisungen einholen.

English:

Obtain special instructions before use.

Français:

Se procurer les instructions avant utilisation.

Italiano:

Procurarsi istruzioni specifiche prima dell’uso.

Español:

Pedir instrucciones especiales antes del uso.

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P202

Deutsch:

Vor Gebrauch sämtliche Sicherheitsratschläge lesen und verstehen.

English:

Do not handle until all safety precautions have been read and understood.

Français:

Ne pas manipuler avant d’avoir lu et compris toutes les précautions de sécurité.

Italiano:

Non manipolare prima di avere letto e compreso tutte le avvertenze.

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No manipular la sustancia antes de haber leído y comprendido todas las instrucciones de seguridad.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P308 +P313

Deutsch:

BEI Exposition oder falls betroffen: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

English:

IF exposed or concerned: Get medical advice/attention.

Français:

EN CAS d’exposition prouvée ou suspectée: consulter un médecin.

Italiano:

IN CASO di esposizione o di possibile esposizione, consultare un medico.

Español:

EN CASO DE exposición manifiesta o presunta: Consultar a un médico.

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P405

Deutsch:

Unter Verschluss aufbewahren.

English:

Store locked up.

Français:

Garder sous clef.

Italiano:

Conservare sotto chiave.

Español:

Guardar bajo llave.