Cellulase »Onozuka R-10®« from Trichoderma viride ca. 1 U/mg

CAS [9012-54-8]

Cellulase Onozuka R-10® is used for the degradation of plant cell walls, usually for the production and isolation of metabolically active protoplasts, often in combination with Macerozyme R-10 (cat. no. 28032) (2).
The cellulase Onozuka R-10® is a multi-component enzyme system (1). Although the preparation has high cellulase activity, it contains hemicellulases and degrades mannans, xylans, galactomannans, pectins and other polysaccharides.
Temperature optimum: 40 - 50 °C
pH-optimum: pH 4 - 5

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).

Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U, protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5, calculated as xylose).
Onozuka R-10® is a registered trademark of Yakult Pharmaceuticals Industry Co., Ltd..


References:
  1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
  2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
  3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
  4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EG-Index: 647-002-00-3EINECS: 232-734-4WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
16419.02
2.5 g
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16419.03
10 g
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16419.05
50 g
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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.