Proteinase K from Tritirachium album min. 8 DMC-U/mg
lyophil.
EC 3.4.21.14 • Mr ca. 28 000 • CAS [39450-01-6]
Serin protease with very broad range of action: cleaves peptide bonds at the
carboxylic side of aliphatic, aromatic, and hydrophobic amino acids.
Suitable for the isolation of DNA and RNA (1, 3).
Unit definition 1 DMC-unit catalyzes the hydrolysis of 1 µmole dimethyl
casein per minute at 25 °C, pH 7.0; the liberated amino groups are determined
with 2,4,6-trinitrobenzene sulfonic acid (2). Activities in other units ca. 30 milliAnson-units. Inhibitors for Proteinase: AEBSF (cat. no. 12745), (PEFABLOC® SC
(cat.no. 31682), PMSF (cat. no. 32395) and Diisopropylfluorophosphate (cat.
no. 77205). Extraneous activities: DNases and RNases not detectable.
References:
Ebeling, W. et al.(1974) Eur. J. Biochem. 47, 91-7
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BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.
