(POD, HRP, Donor: hydrogen-peroxide oxidoreductase) EC 1.11.1.7 • Mr ca. 40 000 • CAS [9003-99-0]
For the determination of peroxide (1). Used as an indicator enzyme in
reactions where peroxide is produced (2). For labelling antibodies in
ELISA (3, 4). RZ (= A 403/A 275) = 3.0.
Unit definition: 1 U catalyzes the decomposition of 1 µmole hydrogen
peroxide per minute at 25 °C, pH 7.0; reaction coupled with phenol-
aminoantipyrine (5). Activity in other units: min. 250 purpurogallin units/mg (1
purpurogallin unit catalyzes the oxidation of 1 mg pyrogallol to purpurogallin
in 20 seconds at 20 °C and pH 6.0. The purpurogallin is extracted and
determined spectrophotometrically at 420 nm (6). 1 mg purpurogallin
requires 13.5 µmole peroxide, 1 purpurogallin unit corresponds to the
decomposition of 12 µmoles peroxide at 25 °C).
References:
Meiattini, F. (1985) Methods of Enzymatic Analysis, (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 7, p. 566-71
Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed.Vol. 2, p. 267-8
Johnson Jr., R.B. (1980) J. Immunoassay 1, 27-37
Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
Gallati, H. (1977) J. Clin. Chem. Clin. Biochem. 15, 699-703
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H334
Deutsch:
Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.
English:
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Français:
Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.
Italiano:
Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Español:
Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.
BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position
ruhigstellen, die das Atmen erleichtert.
English:
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
Français:
EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.
Italiano:
IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
Español:
EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.
