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Nitro Blue tetrazolium chloride
analytical grade
(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye)
C40H30Cl2N10O6 • Mr 817.7 • CAS [298-83-9]
C40H30Cl2N10O6 • Mr 817.7 • CAS [298-83-9]
A ditetrazolium compound for demonstrating succinic dehydrogenase activity (1). Glucose-6-phosphate dehydrogenase (2). Xanthine oxidase (3). Used in conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase.
Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.
Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2,
100 mM Tris; pH 9.5)
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Purity
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min. 98.0 %
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References:
- Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
- Negi, D.S. & Stephens, R.J. (1977) J. Histochem. Cytochem. 25, 149-54
- Auscher, C. & Amory, N. (1976) Biomedicine 5, 37-8
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| WARNING |
| Hazard Statements | H302 - H332 |
| Precautions | P261 |
| Reaction | P301 +P312 - P304 +P340 - P330 |
| Storage | P501 |
EINECS: 206-067-4 • WGK: 1 • HS: 29339980
Storage Temperature: +2 °C to +8 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 30550.01 | 250 mg | inquire* | |
| 30550.02 | 1 g | inquire* | |
| 30550.03 | 5 g | inquire* | |
| *Please log in to see your actual prices. | |||
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