(NBT, Nitro BT, Nitrotetrazolium blue chloride, Ditetrazolium dye) C40H30Cl2N10O6 • Mr 817.7 • CAS [298-83-9]
A ditetrazolium compound for demonstrating succinic dehydrogenase activity
(1). Glucose-6-phosphate dehydrogenase (2). Xanthine oxidase (3). Used in
conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase. Stock
solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C. Staining
solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock
solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100
mM Tris; pH 9.5)
min. 98.0 %
Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
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ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.
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