(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye) C40H30Cl2N10O6 • Mr 817.7 • CAS [298-83-9]
A ditetrazolium compound for demonstrating succinic dehydrogenase activity
(1). Glucose-6-phosphate dehydrogenase (2). Xanthine oxidase (3). Used in
conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase. Stock
solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C. Staining
solution for western blots: 66 µl NBT stock solution and 33 µl BCIP stock
solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100
mM Tris; pH 9.5)
Purity
min. 98.0 %
References:
Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
Proteomics in Practice (Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt. Electrophoresis in Practice Reiner Westermeier): 109,00 € inkl. 7 % MWSt.
Proteomics in Practice: (HPE-PIP) Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Electrophoresis in Practice: (HPE-EIP) Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.
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