SERVA Electrophoresis GmbH › Enzymology › Enzymes › Neutral Protease NB from Clostridium histolyticum
 

Reagents A-Z
SERVA Catalog
Sample Preparation Collagenase & Neutral Protease

For Tissue Dissociation

Electrophoresis

From Sample Preparation to Image

Enzymology

Enzymes, Substrates, Inhibitors, Detection

Ion Exchange Media

Purification of Biomolecules

Life Sciences

From Cells to Molecules

Microscopy

Fixation, Embedding, Staining

 

Partner Catalogs
Hoefer Inc., USA

Equipment for Electrophoresis

Elchrom Scientific, CH

Equipment & Gels for DNA Electrophoresis

BioCision, USA

Standardizing Samples

Delphi™ Genetics SA

Cloning & Expression
in Bacteria

IMPLEN GmbH, D

NanoPhotometer
for Small Volumes

Isogen Life Science B.V.

Proxima Imaging Platform

Generon Ltd., UK

GST-tagged Protein Purification

Pro-Chem Inc., USA

Antibody Purification

 

HomeEnzymologyEnzymes › Neutral Protease NB from Clostridium histolyticum

Neutral Protease NB from Clostridium histolyticum

Mr ca. 34 000CAS [9001-92-7]

Contains chromatographically purified neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Largely free of collagenolytic activity.
Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade (cat. no. 17455) as well as Collagenase NB 4 Standard Grade (cat. no. 17454) and Collagenase NB 8 Broad Range (cat. no. 17456).
For research use only. If cells are intended for transplantation into humans,
Neutral Protease NB GMP Grade (cat. no. 30303) is recommended.

Unit definition: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).


References:
  1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
DANGER
Hazard Statements H315 - H317 - H319 - H334 - H335
Precautions P280
Reaction P302 +P352 - P304 +P341 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-014-00-9EINECS: 232-642-4WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C


Cat.No. Size EUR
30301.11 50 DMC-U inquire*  
30301.12 100 DMC-U inquire*  
*Please log in to see your actual prices.

PDF Downloads 
Specifications
Sicherheitsdatenblatt
ManualnullTechnical Notes


Additional Information 
Protocol for isolation of pancreatic islets from pig
SERVA Statement TSE safety.pdf

Tuesday, May 21, 2013

Shop Login




Product Inquiry
Name

E-Mail

Product

Message



SERVA News

SERVA Promotions V/2013


 

Große Frühjahrs-Aktion!
Sonderaktionen (D + A)

Promotions (EU)

SERVA Elektrophorese-Tage 2013


Buy now from SERVA!


Proteomics in Practice
(Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt.

Electrophoresis in Practice
Reiner Westermeier): 109,00 € inkl. 7 % MWSt.

 more

Now available: New Brochure Protein Analysis - Sample Preparation!


 more

New Brochure Protein Analysis - Everygel for Electrophoresis!


 more

ICPL™ Quadruplex Plus Kit -


 more

SERVA Promotions V/2013

Close window

 

Große Frühjahrs-Aktion!
Sonderaktionen (D + A)

Promotions (EU)

Promotions_Eichhorn.jpg

SERVA Elektrophorese-Tage 2013

Close window

Seminartermine/-Orte
Online-Reservierung

SET13.jpg

Buy now from SERVA!

Close window

Proteomics in Practice
(Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker): 99,90 € inkl. 7 % MWSt.

Electrophoresis in Practice
Reiner Westermeier): 109,00 € inkl. 7 % MWSt.

Westermeier_books.jpg

Proteomics in Practice:
(HPE-PIP)
Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.

Electrophoresis in Practice:
(HPE-EIP)
Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.


HPE-PIP
HPE-EIP
Buy now from SERVA

Now available: New Brochure Protein Analysis - Sample Preparation!

Close window

Sample-Preparation_Brochure.jpg

From Sample Preparation to Image:


SERVA Brochure: Sample Preparation (pdf)

New Brochure Protein Analysis - Everygel for Electrophoresis!

Close window

Everygel_Brochure_s.jpg

From Sample Preparation to Image:


SERVA Brochure: Everygel for Electrophoresis (pdf)

ICPL™ Quadruplex Plus Kit -

Close window

ICPL-Kit.jpg

Now with improved sequence coverage by protein double digestion!


SERVA ICPL™ Quadruplex Plus Kit 39233

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.

H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

close

H317

Deutsch:

Kann allergische Hautreaktionen verursachen.

English:

May cause an allergic skin reaction.

Français:

Peut provoquer une allergie cutanée.

Italiano:

Può provocare una reazione allergica cutanea.

Español:

Puede provocar una reacción alérgica en la piel.

close

H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

close

H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

close

H335

Deutsch:

Kann die Atemwege reizen.

English:

May cause respiratory irritation.

Français:

Peut irriter les voies respiratoires.

Italiano:

Può irritare le vie respiratorie.

Español:

Puede irritar las vías respiratorias.

close

P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

close

P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

close

P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

close

P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

close

P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.

close