(»Macerating Enzyme«, »Cell Separating Enzyme«) EC 3.2.1.15 • CAS [9032-75-1]
Macerozyme R-10 is suitable for the isolation of plant cells, and can be used
in combination with cellulase »Onozuka R-10« (cat. no. 16419) (1, 2) and with
Cellulase »Onuzuka RS« (cat. no. 16420).
Activities: Pectinase ca. 0.5 U/mg Unit definition: 1 U
catalyzes the liberation of 1 µmole of reducing groups from pectic acid per
minute at 25 °C, pH 4.5 calculated as galacturonic acid. Reducing groups
determined with alkaline copper reagent (2). Hemicellulase: ca. 0.25
U/mg Unit definition: 1 U is equivalent to 1 µmole of reducing
groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as
xylose. Cellulase: ca. 0.1 U/mg Unit definition: 1 U
catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl
cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper
reagent (3). pH-optimum: 3.5 - 7.0 Temperature optimum: 40
- 50 °C.
References:
Yamada, Y. et al. (1972) Agr. Biol. Chem. 36, 1055-9
Barraclough, R. & Ellis, R.J. (1979) Eur. J. Biochem. 94, 165-77
Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
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H334
Deutsch:
Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.
English:
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Français:
Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.
Italiano:
Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Español:
Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.
BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position
ruhigstellen, die das Atmen erleichtert.
English:
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
Français:
EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.
Italiano:
IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
Español:
EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.
