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Crystalline powder in hydrochloride form. Lysozyme hydrolyzes the
β 1 → 4 linkages of the murein between N-acetylmuramic acid and N-acetyl-D-glucosamin, and degrades the heteroglycan chain to disaccharides. This reaction leads to cell lysis in most grampositive bacteria e.g. Micrococcus luteus. Lysis can be prevented if the reaction is performed in an isotonic sucrose medium. Under these assay conditions protoplasts are produced which no longer have a cell wall. The lysis of Micrococcus luteus cells is used for the activity testing of lysozyme. In gramnegative bacteria a compact lipopolysaccharide layer on the exposed murein sacculus efficiently shields them from lysozyme digestion. Only when the stabilizing Ca²⁺ ions are removed by treatment with e.g. EDTA, the murein becomes susceptible to lysozyme. Suitable for hydrolysis of bacterial cell walls and of proteoglycans (1, 2, 4).

Unit definition: 1 unit catalyzes a decrease in absorption at 450 nm of 0.001 per minute at 25 °C, pH 7.0, in a suspension of Micrococcus luteus cells (reaction volume 3 ml, light path 1 cm) (3).
Isoelectric point: 10.5 - 11.0
Optimum pH: 9.2


References:

1. Imoto, T. et al. (1972) The Enzymes VII, 3rd Ed. (Boyer, P.D., ed.) Acad. Press N.Y. 666-70
2. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (1.22, 1.34, 1.36, 1.38, 17.38, 1.29, B.17)
3. Shugar, D. (1952) Biochim. Biophys. Acta 8, 302-9
4. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EINECS: 235-747-3 • HS: 35079090
Storage Temperature: +2 °C to +8 °C

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Thursday, May 24, 2012

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