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Isopropyl-β-D-thiogalactopyranoside
research grade
(IPTG, Isopropyl-1-thio-β-D-galactopyranoside)
C9H18O5S • Mr 238.3 • CAS [367-93-1]
C9H18O5S • Mr 238.3 • CAS [367-93-1]
Analog of galactose, not split by β-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with XGal
(5-bromo-4-chloro-indolyl-β-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.
|
Assay (HPLC) [α] 20 °C/D (c=1 in water) Dioxane |
min. 99 % -30.5 ° to -32.5 ° max. 5 ppm |
References:
- Donner, J. et al. (1982) J. Biol. Chem. 257, 14826-9
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (4.33, 4.37-4.38, 1.8-1.9, 17.12-17.13, B.11)
- Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (1.4.3, 16.2.3, 1.15.1)
EINECS: 206-703-0 • WGK: 1 • HS: 29389090
Storage Temperature: -15 °C to -25 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 26600.03 | 1 g | inquire* | |
| 26600.04 | 10 g | inquire* | |
| 26600.06 | 50 g | inquire* | |
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