LavaPurple™ is based on a small naturally occurring fluorescent compound (1)
that
reversibly binds to lysine, arginine, and histidine residues in proteins and
peptides (2)
to yield an intensely red-fluorescent product. This unique mechanism provides
sensitive quantification of proteins in 1 and 2D gels of all chemistries, on
both PVDF
and nitrocellulose blots (3, 4) and provides unparalleled compatibility with
mass
spectrometry (5).
LavaPurple = registered trademark of Fluorotechnics
References:
Bell, P.J.L. and Karuso, P. (2003) Epicocconone, a novel fluorescent compound
from the fungus Epicoccum nigrum. Journal of the American Chemical
Society. 125, 9304
Coghlan, D. R., Mackintosh, J. & Karuso, P. (2005). Mechanism of reversible
fluorescent staining of protein with Epicocconone. Organic Letters. 7,
2401-240
Malmport, E., Mackintosh, J., Ji, H., Veal, D. & Karuso, P. (2005).
Visualization of proteins electro-transferred on Hybond ECL and Hybond-P using
Deep Purple Total Protein Stain. GEHealthcare Life Science News. 19,
12-13
Mackintosh, J.A., Choi, H.-Y., Bae, S.-H., Veal, D.A., Bell, P.J., Ferrari,
B.C., van Dyk, D., Verrills, N.M., Paik, Y.-K. & Karuso, P. (2003). A
fluorescent natural product for ultra sensitive detection of proteins in 1-D
and 2-D gel electrophoresis. Proteomics. 3, 2273-2288
Tannu, N.S. Sanchez Brambila, G.S., Kirby, P., Andacht, T.M. (2006) Effect of
staining reagent on peptide mass fingerprinting from in-gel trypsin
digestions: A comparison of Sypro Ruby and Deep Purple.Electrophoresis 27,
3136 - 314
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