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DNA Polymerase I Klenow Fragment, 3 U/µl
sequencing grade
Mr
68 000
• CAS [9012-90-2]
Solution in in 100 mM Tris-HCl (pH 7.5), 10 mM 2-mercaptoethanol and 50 % glycerol. RNases, Nickases, Exo- and Endonucleases not detected.
Supplied with 10 x Assay Buffer: 500 mM Tris-HCl (pH 7.6 at 25 °C), 100 mM MgCl2.
The large fragment of DNA polymerase I, Klenow Fragment, has two of the three properties of the intact DNA polymerase I enzyme. Klenow Fragment has 5’ → 3’ DNA polymerase activity and 3’ → 5’ exonuclease activity. However it lacks the 5’ → 3’ exonuclease activity of intact DNA polymerase I.
Unit Definition: One unit is defined as the amount of enzyme required to convert 10 nmoles of total deoxyribonucleotides to an acid insoluble form in 30 minutes at 37 °C.
EINECS: 232-741-2 • HS: 38220000
Storage Temperature: -15 °C to -25 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 39718.01 | 250 U | inquire* | |
| *Please log in to see your actual prices. | |||
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