Online Shop Login

The SERVA BluePrep 4in1 Purification Kit provides a fast and efficient procedure for isolation and purification of total RNA, including microRNA, genomic DNA and proteins sequentially from a single sample. By using the provided microRNA enrichment column the separate purification of small RNA (<200nt) is possible, allowing for the purification of large RNA, microRNA, genomic DNA and proteins from one single sample. Samples from a broad range of sources like animal cells, tissues (plant, animal and human), blood, bacteria, yeast and fungi can be processed. Because the method bases on spin column chromatography, no phenol/chloroform extraction and acetone precipitation of proteins are necessary. It is the ideal kit for researchers interested in analysis of the proteome and transcriptome of a single sample for studies of gene expression (e.g. gene silencing, mRNA knockdowns), biomarker discoveries, studies in epigenetics and for characterization of cell lines. No need for fractionation of the sample, which reduces variability and inconsistent results and saves material from difficult to obtain or small samples. Process up to 10 samples in only 40 minutes (separation of microRNA included).
The purified RNA is highly integer and suitable for a number of downstream applications like real time PCR, RT-PCR, Northern Blotting, RNase protection assays, primer extension and expression arrays. The purified proteins can be used in all common applications such as Western Blotting, SDS-PAGE, and IEF etc.

Kit specifications:

Column binding capacity: RNA 50 µg, DNA 200 µg, protein 200 µg

Column reservoir capacity: 600 µl

Size of RNA purified: all sizes (separate purification of miRNA, siRNA optional)

Size of DNA purified: > 30 kb

Average yield (HeLa Cells, 1 x 10

⁶ cells): 15 µg RNA, 8 µg DNA, 150 µg protein
Maximum amount of starting material:

Animal cells Animal tissues Blood Bacteria Yeast Fungi Plant tissues

3 x 106 cells 10 – 20 mg 100 µl 1 x 109 cells 1 x 108 cells 50 mg 50 mg

HS: 38220000

PDF Downloads 

Wednesday, May 23, 2012

Home    Disclaimer    Print Version