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SERVA BluePrep IB Isolation Macro Kit

4 reactions

The SERVA BluePrep IB Isolation Macro Kit facilitates the preparation of recombinant proteins from E. coli inclusion bodies from large culture volume. The kit provides reagents for fast and high-quality purification of inclusion bodies using three steps: 1. Lysis of bacterial cells to release inclusion bodies 2. Solubilization of purified inclusion bodies 3. Purification of the recombinant protein using spin column cromatography based on ion exchange.
The gentle cell disruption procedure provides a highly pure final product (often > 95 %). Process 1 sample in only 60 minutes.
The final elution is performed with choices of several different buffers suitable for:
  • Mass spectrometry
  • SDS-PAGE
  • 2 D-PAGE
  • Refolding experiments


  • Kit specifications:
    Maximum culture volume
    Yield from 100 ml
    Minimum elution volume
    100 ml
    up to 12 mg
    4 ml


    HS: 38220000



    Cat.No. Size EUR
    42078.01 4 reactions inquire*  
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    Wednesday, May 23, 2012

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