Single tube format assay kit for protein quantification. The assay bases on
the precipitation of proteins as insoluble dye complexes with acidic,
ethanolic amido black 10B solution (1,2). After precipitation the protein-dye
complexes are spinned down. The pellet is washed and resolubilized. The
thereby released dye amount is measured at 624 nm.
Precise, reproducible, reliable assay data
Completed in only 45 min.
No interference with detergents or reducing agents
Detection range starts as low as 2 µg protein.
References:
Schaffner W., Weissmann C., A rapid, sensitive, and specific method for the determination
of protein in dilute solution, Anal. Biochem. 1973; 65: 502-514.
Popov N., Schmitt M., Schulzeck S., Matthies H., Reliable micro method for
determination of the protein content in tissue homogenates, Acta Biol. Med.
Ger. 1975; 34 (9): 1441-1446.
For his work on a new selective method to isolate and analyze phospho- and glycoproteins Fabio Polato from the University of Innsbruck received the SERVA Young Scientists Award 2010 during the "17. Arbeitstagung Mikromethoden in der Proteinchemie" (21 to 23 June 2010, Martinsried, Germany). Congratulations!
(in front: Fabio Polato (Universität Innsbruck) and Barbara Müller (SERVA, Heidelberg), background left: Helmut E. Meyer (Ruhr-Universität Bochum), right: Roland Kellner (MERCK, Darmstadt)
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