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Proteinase K from Tritirachium album
solution 20 mg/ml, min. 30 miliAnson-U/mg
EC 3.4.21.14 • Mr
Mr 28.000
•
Serine protease with very broad range of action: cleaves peptide bonds at the carboxylic side of aliphatic, aromatic, and hydrophobic amino acids.
Suitable for the isolation of DNA and RNA (1, 3).
Unit definition: 1 unit is defined as the amount of enzyme that liberates Folin-positive amino acids and peptides, corresponding to 1 µmol tyrosine per minute at 37 °C and pH 7.4 using urea-denatured hemoglobin as substrate (4)
Activities in other units: min. 8 DMC-U/mg. One DMC-Unit is that amount of enzymatic activity which catalyzes the cleavage of 1 µmole equivalent peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of the appearance of new terminal amino groups.
Inhibitors for Proteinase: AEBSF (cat. no. 12745), (PEFABLOC® SC (cat.no. 31682), PMSF (cat. no. 32395) and Diisopropylfluorophosphate (cat. no. 77205).
Extraneous activities: DNases and RNases not detectable.
References:
- Ebeling, W. et al.(1974) Eur. J. Biochem. 47, 91-7
- Lin, Y. et al.(1969) J. Biol. Chem. 244, 789-93
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.16, 1.61)
- Anson, M.L. (1938) J. Gen. Physiol. 33, 79-89
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| DANGER |
| Hazard Statements | H334 |
| Precautions | P261 - P285 |
| Reaction | P304 +P341 - P342 +P311 |
WGK: 1 • HS: 38220000
Storage Temperature: -15 °C to -25 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 33755.01 | 1 ml | inquire* | |
| 33755.02 | 5 ml | inquire* | |
| 33755.03 | 10 ml | inquire* | |
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