96-well format assay kit for protein quantification. The kit bases on the
precipitation of proteins as insoluble dye complexes with acidic, methanolic
amido black 10B solution (1, 2). After precipitation the protein-dye complexes
are spinned down. The pellet is washed and resolubilized. The thereby released
dye amount is measured at 620 nm. Linearity from 100 to 1750 µg/ml protein. In
triple measurement 23 samples can be analysed (plus BSA standard and blank). Note:
Ampholytes bind to the dye as well and therefore simulate too high protein
concentrations. Protein measurement of analytic solutions for 2DGE must be
done before ampholyte addition.
Precise, reproducible, reliable assay
data
Completed in 45 min.
No interference with detergents or
reducing agents
High-throughput format (96-well plate)
Linear
detection range from approx. 100 - 1750 µg/ml protein
The kit
contains 10x dye concentrate, elution solution, bovine serum albumin as
standard reference, 96-well plates.
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Schädigt die Organe <oder alle betroffenen Organe nennen, sofern bekannt> <Expositionsweg angeben, sofern schlüssig belegt ist, dass diese Gefahr bei keinem anderen Expositionsweg besteht>.
English:
Causes damage to organs <or state all organs affected, if known> <state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard>.
Français:
Risque avéré d'effets graves pour les organes <ou indiquer tous les organes affectés, s'ils sont connus> <indiquer la voie d'exposition s'il est formellement prouvé qu'aucune autre voie d'exposition ne conduit au même danger>.
Italiano:
Provoca danni agli organi <o indicare tutti gli organi interessati, se noti> <indicare la via di esposizione se è accertato che nessun'altra via di esposizione comporta il medesimo pericolo>.
Español:
Provoca daños en los órganos <o indíquense todos los órganos afectados, si se conocen> <indíquese la vía de exposición si se ha demostrado concluyentemente que el peligro no se produce por ninguna otra vía>.
BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.
