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Collagenase NB 4 Standard Grade from Clostridium histolyticum
(Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Contains class I and class II collagenase and a balanced ratio of proteolytic
activities. PZ activity (Wünsch): ≥ 0.1 U/mg Suitable for
different applications in tissue dissociation such as isolations of human and
rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts,
cells from adipose tissue (e.g. ADSC's), stem cells and rodent osteoblasts. As
Collagenase NB 4 Standard Grade possesses comparable enzymatic properties to
Collagenase NB 5 Sterile Grade (cat. no. 17459) and Collagenase NB 6 GMP Grade
(cat. no. 17458), this enzyme might also be used in order to establish
applications which have to meet sterile or cGMP requirements like tissue
engineering and transplantations.
Unit definition: 1 U according to Wünsch (1) catalyzes the hydrolysis of 1
µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine
per minute at 25 °C, pH 7.1.
References:
Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
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