SERVA Electrophoresis › Enzymology › Enzymes › Collagenase NB 4 Standard Grade from Clostridium histolyticum
 
Online Shop Login


HomeEnzymologyEnzymes › Collagenase NB 4 Standard Grade from Clostridium histolyticum

Collagenase NB 4 Standard Grade from Clostridium histolyticum

(Clostridiopeptidase A, Collagenase A)
EC 3.4.24.3Mr ca. 70 000 - 120 000 (collagenases)CAS [9001-12-1]

Contains class I and class II collagenase and a balanced ratio of proteolytic activities.
PZ activity (Wünsch): ≥ 0.1 U/mg
Suitable for different applications in tissue dissociation such as isolations of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSC's), stem cells and rodent osteoblasts.
As Collagenase NB 4 Standard Grade possesses comparable enzymatic properties to Collagenase NB 5 Sterile Grade (cat. no. 17459) and Collagenase NB 6 GMP Grade (cat. no. 17458), this enzyme might also be used in order to establish applications which have to meet sterile or cGMP requirements like tissue engineering and transplantations.

Unit definition: 1 U according to Wünsch (1) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.


References:
  1. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
  2. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
DANGER
Hazard Statements H315 - H317 - H319 - H334 - H335
Precautions P280 - P285
Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EINECS: 232-582-9WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C


Cat.No. Size EUR
17454.02 500 mg inquire*  
17454.01 1 g inquire*  
*Please log in to see your actual prices.

PDF Downloads 
Specifications
Sicherheitsdatenblatt
ManualnullTechnical NotesTechnical Notes


Additional Information 
Collagenases for Tissue Dissociation - General Information
Protocol for isolation of human adipocytes (adult)
Protocol for isolation of human pre-adipocytes
Oocyte Preparation
SERVA Statement TSE safety.pdf

Sunday, February 12, 2012

Shop Login




Product Inquiry
Name

E-Mail

Product

Message



SERVA News

SERVA HPE™ Staining Kits:
Sensitive Proteinfärbung leicht gemacht!


* For all SERVA customers within the EU. Outside Germany and Austria online shop orders only  more

Reagenzien-Aktion I/2012


* For SERVA customers in Germany and Austria only

 more

New Microscopy Brochure!


 more

ICPL™ Quadruplex Plus Kit -


Now with improved sequence coverage by protein double digestion!  more

SERVA Catalog 2010/11 -
valid through 2012


 more

SERVA HPE™ Staining Kits:
Sensitive Proteinfärbung leicht gemacht!

Close window

* For all SERVA customers within the EU. Outside Germany and Austria online shop orders only

POM_II.jpg
43395 SERVA HPE Silver Staining Kit
43396 SERVA HPE Coomassie Staining Kit
Product of the Month February 2012

Reagenzien-Aktion I/2012

Close window

* For SERVA customers in Germany and Austria only

Reagenzen-Aktion.jpg

Erfolgreiche Experimente beginnen bereits bei den Reagenzien !


SERVA Reagenzien-Aktion I/2012

New Microscopy Brochure!

Close window

Microscopy_Brochure.jpg

Now available!
New Brochure Microscopy:
Reagents for Light and Electron Microscopy


SERVA Microscopy Brochure (pdf)

ICPL™ Quadruplex Plus Kit -

Close window

Now with improved sequence coverage by protein double digestion!

ICPL-Kit.jpg
SERVA ICPL™ Quadruplex Plus Kit 39233

SERVA Catalog 2010/11 -
valid through 2012

Close window

SERVA-Katalog-2010-Titel_s_1.jpg
Get here your personal copy!

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.

H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

close

H317

Deutsch:

Kann allergische Hautreaktionen verursachen.

English:

May cause an allergic skin reaction.

Français:

Peut provoquer une allergie cutanée.

Italiano:

Può provocare una reazione allergica cutanea.

Español:

Puede provocar una reacción alérgica en la piel.

close

H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

close

H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

close

H335

Deutsch:

Kann die Atemwege reizen.

English:

May cause respiratory irritation.

Français:

Peut irriter les voies respiratoires.

Italiano:

Può irritare le vie respiratorie.

Español:

Puede irritar las vías respiratorias.

close

P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

close

P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

close

P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

close

P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

close

P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

close

P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.

close