Home › Enzymology › Enzyme Inhibitors › Acarbose from Actinoplanes sp. ca. 100 FIP-AIU/mg

Acarbose from Actinoplanes sp. ca. 100 FIP-AIU/mg

contact dried, amorph

(O-4,6-(dideoxy-4-[[[1S-(1α, 4α, 5β, 6α)]-4,5,6-trihydroxy-3-(hydroxymethyl)-2-cyclohexene-1-yl]amino]-α-D-glucopyranosyl-(1→4)-O-α-D-glucopyranosyl-(1→4)-D-Glucose)
C₂₅H₄₃O₁₈N • M_r 645.6 • CAS [56180-94-0]

Ca. 100 FIP-AIU/mg (FIP-α-amylase inhibitor unit) using porcine pancreatic α-amylase.
This highly purified Acarbose, a pseudotetrasaccharide, inhibits α-amylases [EC 3.2.1.1], α-glucosidases [EC 3.2.1.3], saccharases [EC 3.2.1.48]. Used e.g. in enzymatic conversions and assays where α-amylases, α-glucosidases or saccharases interfere with the reaction.

Purity (HPLC)
Solubility in water
Microbiological examination:
total aerobic microbial count (TAMC)
total combined yeasts/mould count (TYMC)s

95.0 - 101.0
1400 g/L at 20 °C

max. 1000 CFU/g
max. 100 CFU/g

Unit definition: 1 FIP-α-amylase inhibitor unit (AIU) is the amount of Acarbose which will inhibit 2 FIP-units of α-amylase by 50 % at pH 6.8 and 25 °C after previous incubation.

References:

Truscheit, E. et al. (1981): Chemistry and Biochemistry of Microbial α-Glucosidase Inhibitors; Angew. Chem. Int. Ed. Engl. 20, 744-761
Bischoff, H. (1994): Pharmacology of α-Glucosidase Inhibition; Eur. J. Clin. Inv. 24, suppl. 3, 3-10